AI-ACCELERATED DRUG DISCOVERY
Available from Reaxense
This protein is integrated into the Receptor.AI ecosystem as a prospective target with high therapeutic potential. We performed a comprehensive characterization of Norrin including:
1. LLM-powered literature research
Our custom-tailored LLM extracted and formalized all relevant information about the protein from a large set of structured and unstructured data sources and stored it in the form of a Knowledge Graph. This comprehensive analysis allowed us to gain insight into Norrin therapeutic significance, existing small molecule ligands, relevant off-targets, and protein-protein interactions.
Fig. 1. Preliminary target research workflow
2. AI-Driven Conformational Ensemble Generation
Starting from the initial protein structure, we employed advanced AI algorithms to predict alternative functional states of Norrin, including large-scale conformational changes along "soft" collective coordinates. Through molecular simulations with AI-enhanced sampling and trajectory clustering, we explored the broad conformational space of the protein and identified its representative structures. Utilizing diffusion-based AI models and active learning AutoML, we generated a statistically robust ensemble of equilibrium protein conformations that capture the receptor's full dynamic behavior, providing a robust foundation for accurate structure-based drug design.
Fig. 2. AI-powered molecular dynamics simulations workflow
3. Binding pockets identification and characterization
We employed the AI-based pocket prediction module to discover orthosteric, allosteric, hidden, and cryptic binding pockets on the protein’s surface. Our technique integrates the LLM-driven literature search and structure-aware ensemble-based pocket detection algorithm that utilizes previously established protein dynamics. Tentative pockets are then subject to AI scoring and ranking with simultaneous detection of false positives. In the final step, the AI model assesses the druggability of each pocket enabling a comprehensive selection of the most promising pockets for further targeting.
Fig. 3. AI-based binding pocket detection workflow
4. AI-Powered Virtual Screening
Our ecosystem is equipped to perform AI-driven virtual screening on Norrin. With access to a vast chemical space and cutting-edge AI docking algorithms, we can rapidly and reliably predict the most promising, novel, diverse, potent, and safe small molecule ligands of Norrin. This approach allows us to achieve an excellent hit rate and to identify compounds ready for advanced lead discovery and optimization.
Fig. 4. The screening workflow of Receptor.AI
Receptor.AI, in partnership with Reaxense, developed a next-generation technology for on-demand focused library design to enable extensive target exploration.
The focused library for Norrin includes a list of the most effective modulators, each annotated with 38 ADME-Tox and 32 physicochemical and drug-likeness parameters. Furthermore, each compound is shown with its optimal docking poses, affinity scores, and activity scores, offering a detailed summary.
Norrin
partner:
Reaxense
upacc:
Q00604
UPID:
NDP_HUMAN
Alternative names:
Norrie disease protein; X-linked exudative vitreoretinopathy 2 protein
Alternative UPACC:
Q00604; B2R8K6; Q5JYH5
Background:
Norrin, also known as Norrie disease protein and X-linked exudative vitreoretinopathy 2 protein, is pivotal in activating the canonical Wnt signaling pathway through FZD4 and LRP5 coreceptors. It plays a crucial role in retinal vascularization, signaling via beta-catenin stabilization and LEF/TCF-mediated transcriptional programs. Norrin acts alongside TSPAN12 to activate FZD4, indicating a Wnt-independent signaling pathway that also promotes beta-catenin accumulation. Its involvement in neural cell differentiation and proliferation, as well as neuroectodermal cell-cell interaction, underscores its biological significance.
Therapeutic significance:
Norrin's association with Norrie disease, characterized by early childhood blindness and potential mental disorders, and Vitreoretinopathy, exudative 2, highlights its therapeutic significance. Understanding Norrin's role in these diseases could lead to innovative treatments targeting the underlying genetic variants, offering hope for patients suffering from these retinal disorders.
