Available from Reaxense
This protein is integrated into the Receptor.AI ecosystem as a prospective target with high therapeutic potential. We performed a comprehensive characterization of Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta including:
1. LLM-powered literature research
Our custom-tailored LLM extracted and formalized all relevant information about the protein from a large set of structured and unstructured data sources and stored it in the form of a Knowledge Graph. This comprehensive analysis allowed us to gain insight into Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta therapeutic significance, existing small molecule ligands, relevant off-targets, and protein-protein interactions.
Fig. 1. Preliminary target research workflow
2. AI-Driven Conformational Ensemble Generation
Starting from the initial protein structure, we employed advanced AI algorithms to predict alternative functional states of Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta, including large-scale conformational changes along "soft" collective coordinates. Through molecular simulations with AI-enhanced sampling and trajectory clustering, we explored the broad conformational space of the protein and identified its representative structures. Utilizing diffusion-based AI models and active learning AutoML, we generated a statistically robust ensemble of equilibrium protein conformations that capture the receptor's full dynamic behavior, providing a robust foundation for accurate structure-based drug design.
Fig. 2. AI-powered molecular dynamics simulations workflow
3. Binding pockets identification and characterization
We employed the AI-based pocket prediction module to discover orthosteric, allosteric, hidden, and cryptic binding pockets on the protein’s surface. Our technique integrates the LLM-driven literature search and structure-aware ensemble-based pocket detection algorithm that utilizes previously established protein dynamics. Tentative pockets are then subject to AI scoring and ranking with simultaneous detection of false positives. In the final step, the AI model assesses the druggability of each pocket enabling a comprehensive selection of the most promising pockets for further targeting.
Fig. 3. AI-based binding pocket detection workflow
4. AI-Powered Virtual Screening
Our ecosystem is equipped to perform AI-driven virtual screening on Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta. With access to a vast chemical space and cutting-edge AI docking algorithms, we can rapidly and reliably predict the most promising, novel, diverse, potent, and safe small molecule ligands of Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta. This approach allows us to achieve an excellent hit rate and to identify compounds ready for advanced lead discovery and optimization.
Fig. 4. The screening workflow of Receptor.AI
Receptor.AI, in partnership with Reaxense, developed a next-generation technology for on-demand focused library design to enable extensive target exploration.
The focused library for Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta includes a list of the most effective modulators, each annotated with 38 ADME-Tox and 32 physicochemical and drug-likeness parameters. Furthermore, each compound is shown with its optimal docking poses, affinity scores, and activity scores, offering a detailed summary.
Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta
partner:
Reaxense
upacc:
P35913
UPID:
PDE6B_HUMAN
Alternative names:
-
Alternative UPACC:
P35913; B7Z9T9; E7ETT3; Q53XN5; Q9BWH5; Q9UD49
Background:
The Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta plays a pivotal role in visual signal transduction. It is essential for the hydrolysis of cGMP, a critical step in the phototransduction pathway. This enzyme's activity is crucial for converting light signals into electrical signals in the retina, facilitating vision. Its involvement in retinal circadian rhythm and visual signal amplification underscores its significance in ocular physiology.
Therapeutic significance:
Given its crucial role in vision, mutations affecting this protein are linked to Retinitis pigmentosa 40 and congenital stationary night blindness. These conditions underscore the protein's therapeutic significance, as understanding its function and the impact of its genetic variants could lead to targeted treatments for these debilitating visual disorders.