Available from Reaxense
This protein is integrated into the Receptor.AI ecosystem as a prospective target with high therapeutic potential. We performed a comprehensive characterization of Guanine nucleotide exchange factor C9orf72 including:
1. LLM-powered literature research
Our custom-tailored LLM extracted and formalized all relevant information about the protein from a large set of structured and unstructured data sources and stored it in the form of a Knowledge Graph. This comprehensive analysis allowed us to gain insight into Guanine nucleotide exchange factor C9orf72 therapeutic significance, existing small molecule ligands, relevant off-targets, and protein-protein interactions.
Fig. 1. Preliminary target research workflow
2. AI-Driven Conformational Ensemble Generation
Starting from the initial protein structure, we employed advanced AI algorithms to predict alternative functional states of Guanine nucleotide exchange factor C9orf72, including large-scale conformational changes along "soft" collective coordinates. Through molecular simulations with AI-enhanced sampling and trajectory clustering, we explored the broad conformational space of the protein and identified its representative structures. Utilizing diffusion-based AI models and active learning AutoML, we generated a statistically robust ensemble of equilibrium protein conformations that capture the receptor's full dynamic behavior, providing a robust foundation for accurate structure-based drug design.
Fig. 2. AI-powered molecular dynamics simulations workflow
3. Binding pockets identification and characterization
We employed the AI-based pocket prediction module to discover orthosteric, allosteric, hidden, and cryptic binding pockets on the protein’s surface. Our technique integrates the LLM-driven literature search and structure-aware ensemble-based pocket detection algorithm that utilizes previously established protein dynamics. Tentative pockets are then subject to AI scoring and ranking with simultaneous detection of false positives. In the final step, the AI model assesses the druggability of each pocket enabling a comprehensive selection of the most promising pockets for further targeting.
Fig. 3. AI-based binding pocket detection workflow
4. AI-Powered Virtual Screening
Our ecosystem is equipped to perform AI-driven virtual screening on Guanine nucleotide exchange factor C9orf72. With access to a vast chemical space and cutting-edge AI docking algorithms, we can rapidly and reliably predict the most promising, novel, diverse, potent, and safe small molecule ligands of Guanine nucleotide exchange factor C9orf72. This approach allows us to achieve an excellent hit rate and to identify compounds ready for advanced lead discovery and optimization.
Fig. 4. The screening workflow of Receptor.AI
Receptor.AI, in partnership with Reaxense, developed a next-generation technology for on-demand focused library design to enable extensive target exploration.
The focused library for Guanine nucleotide exchange factor C9orf72 includes a list of the most effective modulators, each annotated with 38 ADME-Tox and 32 physicochemical and drug-likeness parameters. Furthermore, each compound is shown with its optimal docking poses, affinity scores, and activity scores, offering a detailed summary.
Guanine nucleotide exchange factor C9orf72
partner:
Reaxense
upacc:
Q96LT7
UPID:
CI072_HUMAN
Alternative names:
-
Alternative UPACC:
Q96LT7; A8K5W0; D3DRK6; G8I0B6; Q6NUS9
Background:
Guanine nucleotide exchange factor C9orf72 plays a pivotal role in cellular processes, including autophagy, endosomal trafficking, and regulation of mTORC1 signaling. It is a key component of the C9orf72-SMCR8 complex, influencing autophagosome maturation and autophagy initiation. Additionally, it impacts motor neuron actin dynamics and axon extension, highlighting its importance in neuronal function.
Therapeutic significance:
The protein is linked to Frontotemporal dementia and/or amyotrophic lateral sclerosis 1, a neurodegenerative disorder. The disease's genetic basis involves a hexanucleotide repeat expansion in C9orf72, leading to nucleolar stress and cell death. Understanding C9orf72's role could unveil new therapeutic strategies for these conditions.